Datasets -> Peromyscus Californicus -> Whole-Brain Nissl -> Metadata

Dataset ID:    160  
Dataset:  DM3540
Species:  Peromyscus californicus
Age:  3 months
Stain:  Nissl
Stain Abbreviation:  
Method:  histo
Embedding:  frozen
Plane:  coronal
Area:  whole-brain
Source:  Rebecca Hao, Natalia Duque-Wilckens, and Brian Trainor
Source URL:
Date Added:  2016-04-13
Resolution:  0.5 microns/pixel
Section Thickness:  40 microns
Number of Sections:  94   -->  [Generate Paths]
Notes:  The California mouse (Peromyscus californicus) is a monogamous species of rodent found along the coastal mountain ranges and eastern Sierra Nevada of California. It is the largest species in its genus with adult body length ranging between 22-29 cm and body mass between 33-54 g. Adults are distinctly bicolored with yellowish brown or gray mixed with black dorsal coloring and a white underside and feet. Captive colonies of California mice have been useful for the study of the neuroendocrine mechanisms of parental behavior, aggression, and behavioral responses to stress.

After fixation each brain was immersed in 20% sucrose in PBS at 4 C overnight. Brains were then rinsed in PBS before freezing on dry ice. Brains were sectioned at 40 um on a cryostat and then every third section was stained from just posterior to the olfactory bulb through the cerebellum. This specimen was a 3 month old male weighing 39.5 g;